Poursamar, S. A., Lehner, A. N., Azami, M., Ebrahimi-Barough, S., Samadikuchaksaraei, A. and Antunes, A. P. M. (2016) The effects of crosslinkers on physical, mechanical, and cytotoxic properties of gelatin sponge prepared via in-situ gas foaming method as a tissue engineering scaffold. Materials Science and Engineering: C. 63, pp. 1-9. 0928-4931.
| Item Type: | Article |
|---|---|
| Abstract: | In this study porous gelatin scaffolds were prepared using in-situ gas foaming, and four crosslinking agents were used to determine a biocompatible and effective crosslinker that is suitable for such a method. Crosslinkers used in this study included: hexamethylene diisocyanate (HMDI), poly(ethylene glycol) diglycidyl ether (Epoxy), glutaraldehyde (GTA), and genipin. The prepared porous structures were analyzed using Fourier Transform Infrared Spectroscopy (FT-IR), thermal and mechanical analysis as well as water absorption analysis. The microstructures of the prepared samples were analyzed using Scanning Electron Microscopy (SEM). The effects of the crosslinking agents were studied on the cytotoxicity of the porous structure indirectly using MTT analysis. The affinity of L929 mouse fibroblast cells for attachment on the scaffold surfaces was investigated by direct cell seeding and DAPI-staining technique. It was shown that while all of the studied crosslinking agents were capable of stabilizing prepared gelatin scaffolds, there are noticeable differences among physical and mechanical properties of samples based on the crosslinker type. Epoxy-crosslinked scaffolds showed a higher capacity for water absorption and more uniform microstructures than the rest of crosslinked samples, whereas genipin and GTA-crosslinked scaffolds demonstrated higher mechanical strength. Cytotoxicity analysis showed the superior biocompatibility of the naturally occurring genipin in comparison with other synthetic crosslinking agents, in particular relative to GTA-crosslinked samples. |
| Additional Information: | A pre-publication version of this article was made available electronically by the publisher on 13 February 2016 |
| Uncontrolled Keywords: | Gas foaming, denaturation temperature, over-crosslinking, natural crosslinker |
| Subjects: |
R Medicine > R Medicine (General) > R856 Biomedical engineering. Electronics. Instrumentation. > R857.T55 Tissue engineering T Technology > TP Chemical technology > TP248.13 Biotechnology |
| Creators: | Poursamar, Seyed Ali, Lehner, Alexander N, Azami, Mahmoud, Ebrahimi-Barough, Somayeh, Samadikuchaksaraei, Ali and Antunes, A Paula M |
| Publisher: | Elsevier |
| Faculties, Divisions and Institutes: |
University Faculties, Divisions and Research Centres - OLD > Research Centre > Institute of Health and Wellbeing > Ageing Research Centre University Faculties, Divisions and Research Centres - OLD > Research Centre > Institute of Health and Wellbeing > Centre for Physical Activity and Chronic Disease University Faculties, Divisions and Research Centres - OLD > Research Group > Institute for Creative Leather Technologies Research Institutes > Institute for Creative Leather Technologies Research Centres > Centre for Health Sciences and Services Research Centres > Centre for Physical Activity and Life Sciences |
| Date: | 1 June 2016 |
| Date Type: | Publication |
| Page Range: | pp. 1-9 |
| Journal or Publication Title: | Materials Science and Engineering: C |
| Volume: | 63 |
| Language: | English |
| DOI: | https://doi.org/10.1016/j.msec.2016.02.034 |
| ISSN: | 0928-4931 |
| Status: | Published / Disseminated |
| Refereed: | Yes |
| URI: | http://nectar.northampton.ac.uk/id/eprint/8219 |
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