Lee, S. P., Green, A. M., Machado, L., Patel, P., Thomas, W., Goodyear, O., Briggs, D., McDonald, D., Marsden, J. and Steven, N. (2005) Infusing advanced cancer patients with tumour antigen-specific CD4+ and CD8+ T cell clones. Poster presented to: Annual Congress of the British Society for Immunology, Harrogate, UK, 06-09 December 2005. (Unpublished)
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Abstract: | Previous attempts to treat human cancer by infusing tumour antigen- specific T cells have highlighted the need to deliver CD4+ T cells to maintain an effective CD8+ T cell response in vivo. We have explored a protocol designed to generate both CD4+ and CD8+ T cell clones for infusing advanced cancer patients. T cells were reactivated in vitro from patients with EBV-associated tumours, melanoma and prostate cancer using DCs pulsed with peptides representing defined epitopes within tumour antigens. Cloning efficiency was significantly improved by pre-selecting specific cells using an interferon capture assay. CD4+ and/or CD8+ T cell clones were successfully generated from 16/27 patients, six of whom were infused with escalating doses of T cells. Treatments were well tolerated, and functional cells were detectable in blood 1-14 days postinfusion, with some cells present for >2 months. T cell clones were heterogeneous both in their ability to recognise tumour lines and in their homing phenotype. This highlights the value of cloning T cell populations which can be screened prior to infusion to select those with sufficient avidity for the target antigen and with the appropriate homing phenotype. |
| Additional Information: | Abstracts published in Immunology, Vol. 116, Suppl s1, 2005 |
| Subjects: |
R Medicine > RM Therapeutics. Pharmacology > RM270 Immunotherapy. Serotherapy R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology Q Science > QR Microbiology > QR180 Immunology |
| Creators: | Lee, S P, Green, A M, Machado, Lee, Patel, P, Thomas, Wendy, Goodyear, O, Briggs, D, McDonald, D, Marsden, J and Steven, Neil |
| Northamptonshire and East Midlands: | Health |
| Faculties, Divisions and Institutes: |
University Faculties, Divisions and Research Centres - OLD > Faculty of Health & Society > Sports, Exercise & Life Sciences University Faculties, Divisions and Research Centres - OLD > Research Centre > Institute of Health and Wellbeing > Ageing Research Centre Faculties > Faculty of Health & Society > Sports, Exercise & Life Sciences Research Centres > Centre for Health Sciences and Services Research Centres > Centre for Physical Activity and Life Sciences |
| Date: | 2005 |
| Date Type: | Presentation |
| Event Title: | Annual Congress of the British Society for Immunology |
| Event Dates: | 06-09 December 2005 |
| Event Location: | Harrogate, UK |
| Event Type: | Conference |
| Language: | English |
| Status: | Unpublished |
| URI: | http://nectar.northampton.ac.uk/id/eprint/6861 |
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