Northampton Electronic Collection of Theses and Research

Identification and quantification of the antimicrobial components of a citrus essential oil vapour

Phillips, C. A., Gkatzionis, K., Laird, K., Score, J., Kant, A. and Fielder, M. D. (2012) Identification and quantification of the antimicrobial components of a citrus essential oil vapour. Natural Product Communications. 7(1), pp. 103-107. 1555-9475.

Item Type: Article
Abstract: The anti-bacterial components of a citrus essential oil vapor were identified as linalool, citral and beta-pinene using a bioautography method and quantified by GC-MS. Essential oil vapor release, monitored in real-time with Atmospheric Pressure Chemical Ionization - MS (APCI-MS), showed differences in the vapor release profile oflimonene, beta-pinene and linalool over 24 hours, while Solid Phase Micro-extraction (SPME) GC-MS demonstrated changes in composition of the vapor at 35 degrees C. Fourteen isolates were tested in vitro for their susceptibility to the EO vapor and to linalool, citral and beta-pinene vapors, both separately and in a mixture containing the three components in the amounts at which they occur in the EO vapor. All eleven Gram-positive strains tested were susceptible to the EO vapor, linalool, citral and beta-pinene vapors separately and the mixture with zones of inhibition of 4.34 cm, 5.32 cm, 5.58 cm, 4.86 cm and 4.68 cm, respectively. Of the three Gram-negative strains tested, Pseudomonas aeruginosa 10145 was resistant to all the vapors. When bacteria inoculated onto stainless steel surfaces were exposed to either the EO vapor or a linalool/citral/beta-pinene vapor mixture there was no significant difference in reduction for the Gram-positive isolates, while the Gram-negative isolates were resistant to both EO vapor and the linalool/citral/beta-pinene mixture.
Subjects: Q Science > QD Chemistry > QD415 Biochemistry
Q Science > QR Microbiology > QR75 Bacteria
Creators: Phillips, Carol A, Gkatzionis, Konstantinos, Laird, Katie, Score, Jodie, Kant, A and Fielder, M D
Publisher: Natural Product Inc.
Faculties, Divisions and Institutes: University Faculties, Divisions and Research Centres - OLD > Faculty of Health & Society
Date: January 2012
Date Type: Publication
Page Range: pp. 103-107
Journal or Publication Title: Natural Product Communications
Volume: 7
Number: 1
Language: English
ISSN: 1555-9475
Status: Published / Disseminated
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