Phillips, C. A., Gkatzionis, K., Laird, K., Score, J., Kant, A. and Fielder, M. D. (2012) Identification and quantification of the antimicrobial components of a citrus essential oil vapour. Natural Product Communications. 7(1), pp. 103-107. 1555-9475.
Item Type: | Article |
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Abstract: | The anti-bacterial components of a citrus essential oil vapor were identified as linalool, citral and beta-pinene using a bioautography method and quantified by GC-MS. Essential oil vapor release, monitored in real-time with Atmospheric Pressure Chemical Ionization - MS (APCI-MS), showed differences in the vapor release profile oflimonene, beta-pinene and linalool over 24 hours, while Solid Phase Micro-extraction (SPME) GC-MS demonstrated changes in composition of the vapor at 35 degrees C. Fourteen isolates were tested in vitro for their susceptibility to the EO vapor and to linalool, citral and beta-pinene vapors, both separately and in a mixture containing the three components in the amounts at which they occur in the EO vapor. All eleven Gram-positive strains tested were susceptible to the EO vapor, linalool, citral and beta-pinene vapors separately and the mixture with zones of inhibition of 4.34 cm, 5.32 cm, 5.58 cm, 4.86 cm and 4.68 cm, respectively. Of the three Gram-negative strains tested, Pseudomonas aeruginosa 10145 was resistant to all the vapors. When bacteria inoculated onto stainless steel surfaces were exposed to either the EO vapor or a linalool/citral/beta-pinene vapor mixture there was no significant difference in reduction for the Gram-positive isolates, while the Gram-negative isolates were resistant to both EO vapor and the linalool/citral/beta-pinene mixture. |
Subjects: |
Q Science > QD Chemistry > QD415 Biochemistry Q Science > QR Microbiology > QR75 Bacteria |
Creators: | Phillips, Carol A, Gkatzionis, Konstantinos, Laird, Katie, Score, Jodie, Kant, A and Fielder, M D |
Publisher: | Natural Product Inc. |
Faculties, Divisions and Institutes: | University Faculties, Divisions and Research Centres - OLD > Faculty of Health & Society |
Date: | January 2012 |
Date Type: | Publication |
Page Range: | pp. 103-107 |
Journal or Publication Title: | Natural Product Communications |
Volume: | 7 |
Number: | 1 |
Language: | English |
ISSN: | 1555-9475 |
Status: | Published / Disseminated |
Related URLs: | |
URI: | http://nectar.northampton.ac.uk/id/eprint/4751 |
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