Northampton Electronic Collection of Theses and Research

Post-self-assembly experimentation on extruded collagen fibres for tissue engineering applications

Zeugolis, D. I., Paul, R. G. and Attenburrow, G. E. (2008) Post-self-assembly experimentation on extruded collagen fibres for tissue engineering applications. Acta Biomaterialia. 4(6), pp. 1646-1656. 1742-7061.

Item Type: Article
Abstract: Extruded collagen fibres have been shown to constitute a biomimetic three-dimensional scaffold with numerous tissue engineering applications. The multi-step fabrication process of this material provides opportunities for further advancements to improve the properties of the final product. Herein we investigated the influence of the post-self-assembly washing baths on the structural, mechanical and thermal properties of these fibres. The surface morphology and the inter-fibre packing were similar for every treatment. The overnight incubation in isopropanol yielded fibres with the highest temperature and energy of denaturation (p < 0.013). Typical s- and j-shape stress–strain curves were obtained for all treatments in the dry and wet state respectively. Rehydration of the fibres resulted in increased fibre diameter (p < 0.006) and reduced stress (p < 0.001), force (p < 0.001) and modulus (p < 0.002) values for every treatment. In the dry state, the alcohol-treated fibres were characterized by the highest stress (p < 0.002) values; whilst in the wet state the Tris–HCl-treated fibres were the weakest (p < 0.006). For every treatment, in both dry and wet state, a strong and inverse relationship between the fibre diameter and the stress at break was observed. Overall, the fibres produced were characterized by properties similar to those of native tissues.
Uncontrolled Keywords: Extruded collagen fibres; post-self-assembly; mechanical properties; thermal properties; structural characteristics
Subjects: R Medicine > R Medicine (General) > R856 Biomedical engineering. Electronics. Instrumentation. > R857.T55 Tissue engineering
T Technology > TP Chemical technology > TP248.13 Biotechnology
Q Science > QP Physiology > QP501 Animal biochemistry > QP552.C6 Collagen
Creators: Zeugolis, D I, Paul, R G and Attenburrow, Geoff E
Publisher: Elsevier
Faculties, Divisions and Institutes: University Faculties, Divisions and Research Centres - OLD > Research Centre > Centre for Research on Leather and Materials Science
Research Institutes > Institute for Creative Leather Technologies
Date: November 2008
Date Type: Publication
Page Range: pp. 1646-1656
Journal or Publication Title: Acta Biomaterialia
Volume: 4
Number: 6
Language: English
ISSN: 1742-7061
Status: Published / Disseminated
Refereed: Yes

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