Northampton Electronic Collection of Theses and Research

Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy

Antunes, A. P. M., Covington, A. D., Petford, N., Murray, T. and Wertheim, D. (2013) Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy. Journal of Microscopy. 251(1), pp. 14-18. 1365-2818.

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[thumbnail of Novel and innovative way of viewing and manipulating layers of the dermal skin in 3-D]
Novel and innovative way of viewing and manipulating layers of the dermal skin in 3-D
Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy.mp4
Information
Abstract:
The properties and performance of collagen-based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three-dimensional (3-D) visualization on a PC and using a 3-D immersive technology system. The 3-D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.
Uncontrolled Keywords:
Bovine, collagen, confocal laser scanning microscopy, dermis, H&E, immersive 3-D technology
Subjects:
Q Science > QD Chemistry > QD415 Biochemistry
Q Science > QH Natural history > QH201 Microscopy
Creators:
Antunes, A. P. M., Covington, A. D., Petford, N., Murray, T. and Wertheim, D.
Publisher:
Blackwell Publishing
Northamptonshire and East Midlands:
Health
Faculties, Divisions and Institutes:
University Faculties, Divisions and Research Centres - OLD > Research Group > Institute for Creative Leather Technologies
University Faculties, Divisions and Research Centres - OLD > University Departments > Directorate
Research Institutes > Institute for Creative Leather Technologies
Date:
July 2013
Date Type:
Publication
Page Range:
pp. 14-18
Journal or Publication Title:
Journal of Microscopy
Volume:
251
Number:
1
Language:
English
DOI:
10.1111/jmi.12052
ISSN:
1365-2818
Status:
Published / Disseminated
Refereed:
Yes
Related URLs:
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