Lee, S. P., Green, A. M., Machado, L., Patel, P., Thomas, W., Goodyear, O., Briggs, D., McDonald, D., Marsden, J. and Steven, N. (2005) Infusing advanced cancer patients with tumour antigen-specific CD4+ and CD8+ T cell clones. Poster presented to: Annual Congress of the British Society for Immunology, Harrogate, UK, 06-09 December 2005. (Unpublished)
- Information
Information
Abstract:
Previous attempts to treat human cancer by infusing tumour antigen-
specific T cells have highlighted the need to deliver CD4+ T cells
to maintain an effective CD8+ T cell response in vivo. We have
explored a protocol designed to generate both CD4+ and CD8+ T
cell clones for infusing advanced cancer patients. T cells were reactivated
in vitro from patients with EBV-associated tumours, melanoma
and prostate cancer using DCs pulsed with peptides
representing defined epitopes within tumour antigens. Cloning efficiency
was significantly improved by pre-selecting specific cells using
an interferon capture assay. CD4+ and/or CD8+ T cell clones were
successfully generated from 16/27 patients, six of whom were infused
with escalating doses of T cells. Treatments were well tolerated, and
functional cells were detectable in blood 1-14 days postinfusion,
with some cells present for >2 months. T cell clones were heterogeneous
both in their ability to recognise tumour lines and in their
homing phenotype. This highlights the value of cloning T cell
populations which can be screened prior to infusion to select those
with sufficient avidity for the target antigen and with the appropriate
homing phenotype.
Additional Information:
Abstracts published in Immunology, Vol. 116, Suppl s1, 2005
Subjects:
Creators:
Lee, S. P., Green, A. M., Machado, L., Patel, P., Thomas, W., Goodyear, O., Briggs, D., McDonald, D., Marsden, J. and Steven, N.
Northamptonshire and East Midlands:
Health
Faculties, Divisions and Institutes:
University Faculties, Divisions and Research Centres - OLD > Faculty of Health & Society > Sports, Exercise & Life Sciences
University Faculties, Divisions and Research Centres - OLD > Research Centre > Institute of Health and Wellbeing > Ageing Research Centre
Faculties > Faculty of Health & Society > Sports, Exercise & Life Sciences
Research Centres > Centre for Health Sciences and Services
Research Centres > Centre for Physical Activity and Life Sciences
University Faculties, Divisions and Research Centres - OLD > Research Centre > Institute of Health and Wellbeing > Ageing Research Centre
Faculties > Faculty of Health & Society > Sports, Exercise & Life Sciences
Research Centres > Centre for Health Sciences and Services
Research Centres > Centre for Physical Activity and Life Sciences
Date:
2005
Date Type:
Presentation
Event Title:
Annual Congress of the British Society for Immunology
Event Dates:
06-09 December 2005
Event Location:
Harrogate, UK
Event Type:
Conference
Language:
English
Status:
Unpublished
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