Northampton Electronic Collection of Theses and Research

Morphological changes in Enterococcus faecium on exposure to citrus essential oils in vapor phase as determined by TEM, SEM and AFM

Laird, K., Score, J., Prime, D. C. and Phillips, C. A. (2012) Morphological changes in Enterococcus faecium on exposure to citrus essential oils in vapor phase as determined by TEM, SEM and AFM. The Journal of Essential Oil Research. 24(2), pp. 181-185. 1041-2905 (print) 2163-8152 (online).

Item Type: Article
Abstract: The antimicrobial effects of essential oils in vapor phase have been previously reported although the mechanism whereby they bring about their effect continues to be investigated. Morphological changes give some indication of the extent of the damage to cells brought about by exposure to essential oils and may provide a means of substantiating the physiological and biochemical changes that are integral to the antimicrobial effect. In this study, the morphological changes that occur during exposure of Enterococcus faecium to a citrus essential oil in vapor phase has been investigated using transmission electron microscopy (TEM), scanning electron microscopy (SEM) and atomic force microscopy (AFM). TEM shows membrane damage, vacuole formation and leakage of cytoplasmic material, SEM shows deformation of shape, whereas AFM, which gives the most information, confirms the deformation, which could be seen even after 15 minutes exposure using this method.
Uncontrolled Keywords: Citrus EO vapor, TEM, SEM, AFM, Enterococcus faecium
Subjects: Q Science > QR Microbiology > QR75 Bacteria
Creators: Laird, Katie, Score, Jodie, Prime, D C and Phillips, Carol A
Publisher: Taylor & Francis
Faculties, Divisions and Institutes: University Faculties, Divisions and Research Centres - OLD > Faculty of Health & Society
Date: March 2012
Date Type: Publication
Page Range: pp. 181-185
Journal or Publication Title: The Journal of Essential Oil Research
Volume: 24
Number: 2
Language: English
DOI: https://doi.org/10.1080/10412905.2012.659525
ISSN: 1041-2905 (print) 2163-8152 (online)
Status: Published / Disseminated
URI: http://nectar.northampton.ac.uk/id/eprint/4221

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